热门搜索: 50072ATAT1, GST-tag Recombinant 40520ATF2, GST-Tag Recombinant 31114GCN5 (727-837), His-tag Recombinant 50074GCN5 (KAT2A), FLAG-tag Recombinant 50071p300 (KAT3B), His-GST-tag Recombinant 11004ACHE, His-Tag Recombinant 50272BCL-2, His-Tag Recombinant 100077BCL2A1, His-Tag Recombinant 100080BCL2L10, His-Tag Recombinant 100091BCL2L2, His-Tag Recombinant 80113Caspase6, His-tag Recombinant 70000Caspase7, His-tag Recombinant 80114Caspase8, His-tag Recombinant 100543GPX4, FLAG-Tag Recombinant 100054MALT1, FLAG-Tag (Mouse) Recombinant 100637MCL-1 His-Tag (Mouse) Recombinant

PRODUCT CLASSIFICATION

产品分类

TECHNICAL ARTICLES

相关文章

产品展示/ Product display

您的位置:首页  /  产品展示  /    /  Kinase  /  78891JAK1 (JH2 Pseudokinase Domain) Inhibitor Screening Assay Kit

JAK1 (JH2 Pseudokinase Domain) Inhibitor Screening Assay Kit

  • 产品型号:78891
  • 厂商性质:生产厂家
  • 更新时间:2024-01-31
  • 访  问  量:116
立即咨询

联系电话:13829799985

详细介绍
Purchase
Description

The JAK1 (JH2 Pseudokinase Domain) Inhibitor Screening Assay Kit is a fluorescence polarization-based assay designed for screening and profiling small molecules that displace the fluorescently labeled probe (JH2 probe 1) from the JH2 pseudokinase domain of JAK1. This kit comes in a convenient 384-well format, with enough recombinant human JAK1 (JH2 Pseudokinase Domain), buffer, and fluorescently labeled JH2 probe 1 for 384 reactions.

This assay requires a fluorescent microplate reader capable of measuring fluorescence polarization (FP) to read the FP signal. For more information on the principles of FP, visit fp_assays.pdf ().

Figure 1: Illustration of the assay principle. The assay is based on the competition between the test compound and the JH2 probe for the purified JAK1 (JH2 Pseudokinase Domain). JH2 probe 1 is incubated with JAK1 (JH2 Pseudokinase Domain) in the presence or absence of inhibitor. When the JH2 probe 1 is bound to the JH2 pseudokinase domain it has high FP due to its restricted movement. A competitive inhibitor prevents the probe from binding to the JH2 pseudokinase domain, therefore most of the probe is in free form and has low FP. Compared to a control without inhibitor, FP decreases proportionally to the inhibitor concentration.

留言询价

留言框

  • 产品:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 省份:

  • 详细地址:

  • 补充说明:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
关注公众号

邮箱:infocn@bpsbioscience.com

传真:

地址:上海静安区

Copyright © 2024 BPS Bioscience Inc.版权所有   备案号:   技术支持:化工仪器网

TEL:13829799985

扫码加微信