PD-L1 (Mouse)/TCR Activator CHO Cell Line

PD-L1 (Mouse)/TCR Activator CHO Cell Line is a CHO-K1 cell line constitutively expressing mouse PD-L1 (Programmed Cell Death 1 Ligａnd 1, CD274, B7 homolog 1 (B7- H1), GenBank accession #NM_021893) and an engineered T cell receptor (TCR) activator. Surface expression of PD-L1 was confirmed by flow cytometry, and the cell line was functionally validated in a mouse PD-1:PD-L1 cell-based assay.

Figure 1: Mechanism of action of PD-L1 (Mouse)/TCR Activator CHO Cell Line in combination with the Mouse PD-1/NFAT reporter-Jurkat cell line. Jurkat T cells expressing NFAT reporter with constitutive expression of mouse PD-1 (mouse PD-1/NFAT Reporter Jurkat Cell Line, BPS Bioscience #79762) act as effector cells. When co-c*ted, TCR complexes on mouse PD-1/NFAT Reporter Jurkat cells are activated by the TCR activator of PD-L1 (Mouse)/TCR Activator CHO Cell Line, resulting in expression of the NFAT luciferase reporter. However, PD-1 and PD-L1 ligation prevents TCR activation and suppresses the NFAT-responsive luciferase activity. This inhibition can be specifically reversed by anti-mouse PD-1 or anti-mouse PD-L1 antibodies. Mouse PD-1/PD-L1 neutralizing antibodies block PD-1:PD-L1 interaction and promote T cell activation, resulting in reactivation of the NFAT responsive luciferase reporter.

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